Part:BBa_K2787014:Design

GFP under C9 orthogonal RBS with orthogonal 16S rRNA BBa_K2787042

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 798
  Illegal NheI site found at 821
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1661
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1675
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 707
  

Design Notes

Two parts were cloned into one plasmid to avoid issues brought by co-transformation.

Source

The part is constructed by restriction cloning BBa_K2787008 and BBa_K2787002 together into pSB1C3 backbone.